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transendothelial electrical resistance teer  (World Precision Instruments)


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    World Precision Instruments transendothelial electrical resistance teer
    Transendothelial Electrical Resistance Teer, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transendothelial+electrical+resistance/pm41950645-333-5-17?v=World+Precision+Instruments
    Average 94 stars, based on 8 article reviews
    transendothelial electrical resistance teer - by Bioz Stars, 2026-07
    94/100 stars

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    (A) Schematic diagram of the anti-miR library screen to identify miRs/anti-miRs that regulate cell-cell contact and barrier properties in ECs. (B) Mouse lung endothelial cells were transduced using a lentiviral-based, miR-neutralizing shRNA library, selected for puromycin resistance, and clonally expanded. Each well represents the neutralization of a single endogenously expressed miR. Cells were then analyzed by trans-endothelial <t>electrical</t> resistance <t>(TEER)</t> by employing an Electric Cell-substrate Impedance Sensing (ECIS) plate reader to examine paracellular barrier properties. (C) Single high-titer miR-control, miR-23b, and anti-miR-23b lentiviruses were generated and stable miR-modulated human lung endothelial cells established. TEER was measured to assess the specific effect of miR-23b (n=12) (D) miR expression profiling was performed by miR specific qPCR analysis of mouse brain endothelial cells (BECs) from postnatal pups and adult brains as well as primary human brain microvascular endothelial cells (HBMEC) and shown as a heatmap (n=3). (E) Human HUVECs were transfected with miR control or anti-miR-23b mimics (oligonucleotides), and (F) stable HBMECs (parental, miR control, and anti-miR-23b) were generated. HUVEC or HBMEC cells were subjected to TEER by ECIS or EVOM3 analysis (n=4). Results shown as percent change SEM ± n = 4 or 8, independent biological replicates, *p < 0.05, **p < 0.01, and ***p < 0.001 by two-tailed Student’s t-test.
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    (A) Schematic diagram of the anti-miR library screen to identify miRs/anti-miRs that regulate cell-cell contact and barrier properties in ECs. (B) Mouse lung endothelial cells were transduced using a lentiviral-based, miR-neutralizing shRNA library, selected for puromycin resistance, and clonally expanded. Each well represents the neutralization of a single endogenously expressed miR. Cells were then analyzed by trans-endothelial electrical resistance (TEER) by employing an Electric Cell-substrate Impedance Sensing (ECIS) plate reader to examine paracellular barrier properties. (C) Single high-titer miR-control, miR-23b, and anti-miR-23b lentiviruses were generated and stable miR-modulated human lung endothelial cells established. TEER was measured to assess the specific effect of miR-23b (n=12) (D) miR expression profiling was performed by miR specific qPCR analysis of mouse brain endothelial cells (BECs) from postnatal pups and adult brains as well as primary human brain microvascular endothelial cells (HBMEC) and shown as a heatmap (n=3). (E) Human HUVECs were transfected with miR control or anti-miR-23b mimics (oligonucleotides), and (F) stable HBMECs (parental, miR control, and anti-miR-23b) were generated. HUVEC or HBMEC cells were subjected to TEER by ECIS or EVOM3 analysis (n=4). Results shown as percent change SEM ± n = 4 or 8, independent biological replicates, *p < 0.05, **p < 0.01, and ***p < 0.001 by two-tailed Student’s t-test.

    Journal: bioRxiv

    Article Title: miR-23b neutralization in brain endothelium promotes blood-brain barrier repair through Wnt/β-catenin dependent and independent mechanisms

    doi: 10.1101/2025.08.20.671398

    Figure Lengend Snippet: (A) Schematic diagram of the anti-miR library screen to identify miRs/anti-miRs that regulate cell-cell contact and barrier properties in ECs. (B) Mouse lung endothelial cells were transduced using a lentiviral-based, miR-neutralizing shRNA library, selected for puromycin resistance, and clonally expanded. Each well represents the neutralization of a single endogenously expressed miR. Cells were then analyzed by trans-endothelial electrical resistance (TEER) by employing an Electric Cell-substrate Impedance Sensing (ECIS) plate reader to examine paracellular barrier properties. (C) Single high-titer miR-control, miR-23b, and anti-miR-23b lentiviruses were generated and stable miR-modulated human lung endothelial cells established. TEER was measured to assess the specific effect of miR-23b (n=12) (D) miR expression profiling was performed by miR specific qPCR analysis of mouse brain endothelial cells (BECs) from postnatal pups and adult brains as well as primary human brain microvascular endothelial cells (HBMEC) and shown as a heatmap (n=3). (E) Human HUVECs were transfected with miR control or anti-miR-23b mimics (oligonucleotides), and (F) stable HBMECs (parental, miR control, and anti-miR-23b) were generated. HUVEC or HBMEC cells were subjected to TEER by ECIS or EVOM3 analysis (n=4). Results shown as percent change SEM ± n = 4 or 8, independent biological replicates, *p < 0.05, **p < 0.01, and ***p < 0.001 by two-tailed Student’s t-test.

    Article Snippet: The Electrical Cell-Substrate Impedance Sensing system (ECIS ZΘ; Applied BioPhysics) was used to monitor transendothelial electrical resistance (TEER) in real time in 96-well plate format (96W20idf PET plates, Applied BioPhysics).

    Techniques: shRNA, Neutralization, Electric Cell-substrate Impedance Sensing, Control, Generated, Expressing, Transfection, Two Tailed Test